Anti-Calumenin antibody (ab241317)
Key features and details
- Rabbit polyclonal to Calumenin
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Calumenin antibody
See all Calumenin primary antibodies -
Description
Rabbit polyclonal to Calumenin -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Xenopus laevis, Zebrafish, Syrian hamster, Orangutan, Xenopus tropicalis
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Immunogen
Synthetic peptide within Human Calumenin aa 1-50. The exact sequence is proprietary.
Database link: O43852 -
Positive control
- IP: HEK-293T whole cell lysate.
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General notes
ab241317 has not performed satisfactorily when used for WB of Calumenin in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Calumenin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241317 was affinity purified using an epitope specific to Calumenin immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Calumenin antibody (ab241317)
Calumenin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241317 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241317 at 1 µg/ml.
Lane 1: ab241317 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Lysate prepared using NETN lysis buffer.
