Anti-BRAF antibody [CL4004] (ab220032)
![Anti-BRAF antibody [CL4004] (ab220032)](https://www.abcam.com/ps/products/220/ab220032/Images/ab220032-277084-anti-braf-antibody-cl4004-western-blot.jpg "Anti-BRAF antibody [CL4004] (ab220032)")
Key features and details
- Mouse monoclonal [CL4004] to BRAF
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-BRAF antibody [CL4004]
See all BRAF primary antibodies -
Description
Mouse monoclonal [CL4004] to BRAF -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chicken, Quail
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Immunogen
Recombinant fragment (proprietary-tag) corresponding to Human BRAF aa 250-450.
Database link: P15056 -
Positive control
- Human prostate cancer, Human fallopian tube, Human breast cancer and Human testis tissues; MOLT4 cell line lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL4004 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-BRAF antibody [CL4004] (ab220032)Anti-BRAF antibody [CL4004] (ab220032) at 1/500 dilution + MOLT4 cell line lysate
Predicted band size: 84 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (ab220032)](https://www.abcam.com/ps/products/220/ab220032/Images/ab220032-277083-anti-braf-antibody-cl4004-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (ab220032)Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human testis tissue labeling B Raf with ab220032 at 1/500 dilution shows cytoplasmic immunoreactivity in seminiferous tubules.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (ab220032)](https://www.abcam.com/ps/products/220/ab220032/Images/ab220032-277082-anti-braf-antibody-cl4004-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (ab220032)Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human breast cancer tissue labeling B Raf with ab220032 at 1/500 dilution shows moderate cytoplasmic immunoreactivity in tumor cells.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (ab220032)](https://www.abcam.com/ps/products/220/ab220032/Images/ab220032-277080-anti-braf-antibody-cl4004-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (ab220032)Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human fallopian tube tissue labeling B Raf with ab220032 at 1/500 dilution shows strong cytoplasmic immunoreactivity in glandular cells.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (ab220032)](https://www.abcam.com/ps/products/220/ab220032/Images/ab220032-277075-anti-braf-antibody-cl4004-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [CL4004] (ab220032)Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human prostate cancer tissue labeling B Raf with ab220032 at 1/500 dilution shows moderate cytoplasmic immunoreactivity in tumor cells.
