Anti-NFkB Inducing Kinase NIK antibody (ab7204)
Key features and details
- Rabbit polyclonal to NFkB Inducing Kinase NIK
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NFkB Inducing Kinase NIK antibody
See all NFkB Inducing Kinase NIK primary antibodies -
Description
Rabbit polyclonal to NFkB Inducing Kinase NIK -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human NFkB Inducing Kinase NIK aa 795-947.
Database link: Q99558
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NFkB Inducing Kinase NIK antibody (ab7204)All lanes : Anti-NFkB Inducing Kinase NIK antibody (ab7204)
Lane 1 : macrophages lysate
Lane 2 : NIK transfected cell lysateWestern blot detection of NIK in 1, macrophage and 2, NIK transfected cells by anti-human NIK.
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Immunocytochemistry/ Immunofluorescence - Anti-NFkB Inducing Kinase NIK antibody (ab7204)ICC/IF image of ab7204 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7204, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
