Antibodies, Proteins, Kits and Reagents for Life Science

324 988 ₸ Product size

100 µl 324 988 ₸ 10 µl 130 665 ₸

Order now and get it on Tuesday March 02, 2021

Anti-DRAK2 antibody [EPR3163Y] (ab108373)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3163Y] to DRAK2
Suitable for: WB, IP, ICC/IF
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-DRAK2 antibody [EPR3163Y]
See all DRAK2 primary antibodies
Description

Rabbit monoclonal [EPR3163Y] to DRAK2
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Jurkat and Ramos cell lysates
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, 49% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3163Y
Isotype

IgG
Research areas

Western blot - Anti-DRAK2 antibody [EPR3163Y] (ab108373)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: DRAK2 knockout HAP1 cell lysate (20 µg)
Lane 3: Ramos cell lysate (20 µg)
Lane 4: Raji cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108373 observed at 42 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab108373 was shown to recognize DRAK2 when DRAK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and DRAK2 knockout samples were subjected to SDS-PAGE. ab108373 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD)preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-DRAK2 antibody [EPR3163Y] (ab108373)

Immunofluorescence staining of Ramos cells with purified ab108373 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108373 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
Western blot - Anti-DRAK2 antibody [EPR3163Y] (ab108373)

All lanes : Anti-DRAK2 antibody [EPR3163Y] (ab108373) at 1/1000 dilution (purified)

Lane 1 : C6 whole cell lysate
Lane 2 : PC-12 whole cell lysate
Lane 3 : NIH/3T3 whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-DRAK2 antibody [EPR3163Y] (ab108373)

All lanes : Anti-DRAK2 antibody [EPR3163Y] (ab108373) at 1/10000 dilution (purified)

Lane 1 : Ramos whole cell lysate
Lane 2 : Raji whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunoprecipitation - Anti-DRAK2 antibody [EPR3163Y] (ab108373)

ab108373 (purified) at 1/40 immunoprecipitating DRAK2 in 10 μg Ramos cell lysate (Lanes 1 and 2, observed at 42 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
Western blot - Anti-DRAK2 antibody [EPR3163Y] (ab108373)

All lanes : Anti-DRAK2 antibody [EPR3163Y] (ab108373) at 1/500 dilution (Unpurified)

Lane 1 : Jurkat cell lysate +TPA
Lane 2 : Ramos cell lysate
Lane 3 : Ramos cell lysate + TPA

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 42 kDa
Anti-DRAK2 antibody [EPR3163Y] (ab108373)

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