Anti-DRAK2 antibody (ab8419)
Key features and details
- Rabbit polyclonal to DRAK2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DRAK2 antibody
See all DRAK2 primary antibodies -
Description
Rabbit polyclonal to DRAK2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide:
CSKRFRFDDSLPNPHE
, corresponding to amino acids 351/365 of Human DRAK2. (Peptide available as ab8454.) -
Positive control
- Wholecell lysate from Jurkat cells An approximately 45 kDa band can be detected.
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General notes
Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP,are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 (for DAP kinase-related apoptosis-inducing protein kinases) (1). DRAKs contain anN-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK2 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK2 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Purification notes
DRAK2 Antibody is Ion exchange chromatography purified. -
Primary antibody notes
Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP,are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 (for DAP kinase-related apoptosis-inducing protein kinases) (1). DRAKs contain anN-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK2 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK2 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues. -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Images
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Western blot - Anti-DRAK2 antibody (ab8419)Lane 1 : Anti-DRAK2 antibody (ab8419) at 1 µg/ml (DRAK2 antibody)
Lane 2 : Anti-DRAK2 antibody (ab8419) at 2 µg/ml (DRAK2 antibody)
All lanes : Raji cell lysate
Predicted band size: 43 kDa
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Western blot - Anti-DRAK2 antibody (ab8419)All lanes : Anti-DRAK2 antibody (ab8419) at 1/500 dilution
Lane 1 : Jurkat whole cell lysate with absence of blocking peptide
Lane 2 : Raji whole cell lysate with absence of blocking peptide
Lane 3 : Jurkat whole cell lysate with presence of blocking peptide
Lane 4 : Raji whole cell lysate with presence of blocking peptide
Predicted band size: 43 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
We are unsure about the nature of the 70 kDa band. However, DRAK2 is autophosphorylated and it is possible that this band corresponds to the phosphorylated form. The fact that the detection of this band is blocked by DRAK2 peptide indicates that it probably is closely related to the DRAK2 protein. -
Immunocytochemistry/ Immunofluorescence - Anti-DRAK2 antibody (ab8419)ab8419 at 10µg/ml staining DRAK2 in Jurkat cells by ICC/IF
