Anti-AK2 antibody [EPR11387(B)] - BSA and Azide free (ab249309)
![Anti-AK2 antibody [EPR11387(B)] - BSA and Azide free (ab249309)](https://www.abcam.com/ps/products/249/ab249309/Images/ab249309-395383-anti-ak2-antibody-epr11387-western-blot-human-knockout-lysate.jpg "Anti-AK2 antibody [EPR11387(B)] - BSA and Azide free (ab249309)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11387(B)] to AK2 - BSA and Azide free
- Suitable for: WB, IHC-P, Flow Cyt
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-AK2 antibody [EPR11387(B)] - BSA and Azide free
See all AK2 primary antibodies -
Description
Rabbit monoclonal [EPR11387(B)] to AK2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293, HL-60 and HepG2 cell lysates.
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General notes
Ab249309 is the carrier-free version of ab157206. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249309 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11387(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AK2 antibody [EPR11387(B)] - BSA and Azide free (ab249309)All lanes : Anti-AK2 antibody [EPR11387(B)] (ab157206) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : AK2 knockout HEK293T cell lysate
Lane 3 : HL-60 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 26 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab157206).
Lanes 1-4: Merged signal (red and green). Green - ab157206 observed at 30 kDa. Red - loading control ab8245 observed at 36 kDa.
ab157206 Anti-AK2 antibody [EPR11387(B)] was shown to specifically react with AK2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266539 (knockout cell lysate ab257825) was used. Wild-type and AK2 knockout samples were subjected to SDS-PAGE. ab157206 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-AK2 antibody [EPR11387(B)] - BSA and Azide free (ab249309)](https://www.abcam.com/ps/products/249/ab249309/Images/ab249309-2-benefits-of-recombinant-antibodies.png)
Anti-AK2 antibody [EPR11387(B)] - BSA and Azide free (ab249309)
