Anti-ERK2 antibody (ab227134)
Key features and details
- Rabbit polyclonal to ERK2
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ERK2 antibody
See all ERK2 primary antibodies -
Description
Rabbit polyclonal to ERK2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chicken, Cow, Dog, Xenopus laevis, Zebrafish -
Immunogen
Recombinant fragment within Human ERK2 (internal sequence). The exact sequence is proprietary.
Database link: P28482 -
Positive control
- WB: HeLa cell extract; HeLa and H1299 whole cell lysates; Mouse brain lysate. IHC-P: Human colon carcinoma and breast carcinoma tissues. IP: HeLa whole cell extract.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ERK2 antibody (ab227134) at 1/1000 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : ERK2 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 41 kDa10% SDS-PAGE gel.
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Paraffin-embedded human colon carcinoma tissue stained for ERK2 using ab227134 at 1/500 dilution in immunohistochemical analysis.
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ERK2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 4 µg ab227134. Western blot was performed from the immunoprecipitate using ab227134 at 1/500 dilution. Anti-rabbit IgG was used as a secondary reagent.
Lane 1: HeLa whole cell extract 30 µg.
Lane 2: Control IP in HeLa whole cell extract with 4 μg of preimmune Rabbit IgG.
Lane 3: ab227134 IP in HeLa whole cell extract.
10% SDS-PAGE.
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All lanes : Anti-ERK2 antibody (ab227134) at 1/1000 dilution
Lane 1 : H1299 (human non-small cell lung carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 41 kDa10% SDS-PAGE gel.
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Paraffin-embedded human breast carcinoma tissue stained for ERK2 using ab227134 at 1/500 dilution in immunohistochemical analysis.
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Anti-ERK2 antibody (ab227134) at 1/5000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 41 kDa10% SDS-PAGE gel.