Protein L Sepharose® (ab193261)
Overview
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Product name
Protein L Sepharose® -
Product overview
Contents:
Supplied as a 50% slurry in 20% Ethanol, >5 mg Protein L/mL of Sepharose® beads.
Features:
Binding capacity of human IgG is greater than 10 mg/mL of gel; high flow rate; low falling off of rProtein L; pH stability 2-10.
These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x g when centrifuging.
Store beads at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:- Purification of monoclonal and polyclonal antibodies.
- Immunoprecipitation.
Sepharose is a registered trademark of GE Healthcare
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Notes
Protein L Sepharose® is prepared by covalently coupling recombinant Protein L (which contains five Ig light chain binding domains) to 6% cross-linked Sepharose® beads. The coupling technique is optimized to give high binding capacity. The capacity of IgG binding is generally greater than 10 mg of human IgG per mL of wet gel. Protein L binds to a wider range of antibody classes than Protein A or Protein G, including human, mouse and rat IgM, IgA, IgE, and IgD, as well as Fab and single chain variable fragments (scFv). Protein L binds only binds to antibodies that contain Κ light chains, which are found in most human and mouse antibodies.
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Tested applications
Suitable for: Purification, IPmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 ml 5 ml 25 ml 100 ml Protein L Sepharose® 1 x 1ml 1 x 5ml 1 x 25ml 1 x 100ml -
Research areas
