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Immunology Adaptive Immunity T Cells Non-CD

Anti-Bcl3 antibody (ab216877)

Price and availability

268 032 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-Bcl3 antibody (ab216877)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Bcl3
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG

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Overview

  • Product name

    Anti-Bcl3 antibody
    See all Bcl3 primary antibodies
  • Description

    Rabbit polyclonal to Bcl3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    Human
    Recombinant fragment
    See all applications and species data
  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Bcl3 overexpression HEK-293T whole cell lysate; recombinant Bcl3 protein; U-87 MG, C2C12 and NIH/3T3 whole cell lysates. ICC/IF: U-87 MG cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • Non-CD
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Bcl3 antibody (ab216877)
    Immunocytochemistry/ Immunofluorescence - Anti-Bcl3 antibody (ab216877)

    U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells stained for Bcl3 (green) using ab216877 at 10 µg/ml overnight at 4ºC in ICC/IF. The secondary antibody is Anti-Rabbit IgG DyLight™ 488 Conjugated Preadsorbed for at 5 μg/ml for 1 hour at room temperature. Cells were fixed in 4% paraformaldehyde.

    The nuclear counterstain is DAPI (blue).

    A: DAPI

    B: Primary followed by Anti-rabbit IgG Dylight™ 488

    C: Merged images

    D: Secondary only control

  • Western blot - Anti-Bcl3 antibody (ab216877)
    Western blot - Anti-Bcl3 antibody (ab216877)
    All lanes : Anti-Bcl3 antibody (ab216877) at 1/1000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : Bcl3 overexpression HEK-293T, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : DyLight™ 488 Conjugated Pre-Adsorbed antibody at 1/20000 dilution

    Predicted band size: 48 kDa



    Dilution buffer and concentration: PBS/0.01% sodium azide

    Observed MW ~48 kDa ubiquitination, phosphorylation

  • Western blot - Anti-Bcl3 antibody (ab216877)
    Western blot - Anti-Bcl3 antibody (ab216877)
    All lanes : Anti-Bcl3 antibody (ab216877) at 1 µg/ml

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 2 : C2C12 (mouse myoblast cell line) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Rabbit secondary HRP antibody at 1/70000 dilution

    Predicted band size: 48 kDa



    Blocking buffer and concentration: 1% Casein

    Dilution buffer and concentration: PBS/0.01% sodium azide

    Observed MW ~47 kDa endogenous protein

  • Western blot - Anti-Bcl3 antibody (ab216877)
    Western blot - Anti-Bcl3 antibody (ab216877)
    Anti-Bcl3 antibody (ab216877) at 1/1000 dilution + Recombinant human Bcl3 at 0.5 µg

    Secondary
    DyLight™ 488 Conjugated Pre-Adsorbed antibody at 1/20000 dilution

    Predicted band size: 48 kDa



    Dilution buffer and concentration: PBS/0.01% sodium azide

    Observed MW ~75 kDa on recombinant protein.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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