Anti-Fibromodulin antibody [EPR23029-174] - BSA and Azide free (ab269866)
![Anti-Fibromodulin antibody [EPR23029-174] - BSA and Azide free (ab269866)](https://www.abcam.com/ps/products/269/ab269866/Images/ab269866-380890-antifibromodulinantibodyepr23029174westernblot.jpg "Anti-Fibromodulin antibody [EPR23029-174] - BSA and Azide free (ab269866)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23029-174] to Fibromodulin - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Fibromodulin antibody [EPR23029-174] - BSA and Azide free
See all Fibromodulin primary antibodies -
Description
Rabbit monoclonal [EPR23029-174] to Fibromodulin - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Recombinant Human Fibromodulin/FMOD protein, Human articular cartilage, Human uterus, Mouse articular cartilage, Rat articular cartilage, Human uterus and Human uterus treated with PNGase F lysates.
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General notes
ab269866 is the carrier-free version of ab267465. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23029-174 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Fibromodulin antibody [EPR23029-174] - BSA and Azide free (ab269866)All lanes : Anti-Fibromodulin antibody [EPR23029-174] (ab267465) at 1/1000 dilution
Lane 1 : Human articular cartilage lysate
Lane 2 : Human uterus lysate
Lane 3 : Human skin lysate
Lane 4 : HuT-78 (human sezary syndrome cutaneous t lymphocyte) whole cell lysate
Lane 5 : Mouse articular cartilage lysate
Lane 6 : Rat articular cartilage lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 5-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 43 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 12623288, 8895216)
Negative control: Hut-78 (PMID: 15741214)
Exposure time: Lane 1 second; Lanes 2-6:3 secondsThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267465).
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![Anti-Fibromodulin antibody [EPR23029-174] - BSA and Azide free (ab269866)](https://www.abcam.com/ps/products/269/ab269866/Images/ab269866-2-benefits-of-recombinant-antibodies.png)
Anti-Fibromodulin antibody [EPR23029-174] - BSA and Azide free (ab269866)
