Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 103 862 ₸

Order now and get it on Tuesday March 16, 2021

Anti-Fibromodulin antibody [EPR23029-174] (ab267465)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23029-174] to Fibromodulin
Suitable for: WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Fibromodulin antibody [EPR23029-174]
See all Fibromodulin primary antibodies
Description

Rabbit monoclonal [EPR23029-174] to Fibromodulin
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Recombinant Human Fibromodulin/FMOD protein, Human articular cartilage, Human uterus, Mouse articular cartilage, Rat articular cartilage, Human uterus and Human uterus treated with PNGase F lysates.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23029-174
Isotype

IgG
Research areas

Western blot - Anti-Fibromodulin antibody [EPR23029-174] (ab267465)

All lanes : Anti-Fibromodulin antibody [EPR23029-174] (ab267465) at 1/1000 dilution

Lane 1 : Human uterus tissue lysate
Lane 2 : Human uterus tissue treated with PNGase F lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 43 kDa
Observed band size: 60-100 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 12623288, 8895216).

Two bands of 46kDa and 40kDa are de-glycosylated forms. The bands of 60-100kDa are glycosylated forms.

Exposure time: 3 minutes
Western blot - Anti-Fibromodulin antibody [EPR23029-174] (ab267465)

All lanes : Anti-Fibromodulin antibody [EPR23029-174] (ab267465) at 1/1000 dilution

Lane 1 : Human articular cartilage lysate
Lane 2 : Human uterus lysate
Lane 3 : Human skin lysate
Lane 4 : HuT-78 (human sezary syndrome cutaneous t lymphocyte) whole cell lysate
Lane 5 : Mouse articular cartilage lysate
Lane 6 : Rat articular cartilage lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 5-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

Predicted band size: 43 kDa
Observed band size: 60-100 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 12623288, 8895216)

Negative control: Hut-78 (PMID: 15741214)

Exposure time: Lane 1 second; Lanes 2-6:3 seconds
Western blot - Anti-Fibromodulin antibody [EPR23029-174] (ab267465)

Anti-Fibromodulin antibody [EPR23029-174] (ab267465) at 1/1000 dilution + Recombinant Human Fibromodulin/FMOD protein (aa75-376) 20ng

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 43 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST Exposure time: 114 seconds
Anti-Fibromodulin antibody [EPR23029-174] (ab267465)

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