Anti-Fibromodulin antibody [EPR23029-174] (ab267465)
![Anti-Fibromodulin antibody [EPR23029-174] (ab267465)](https://www.abcam.com/ps/products/267/ab267465/Images/ab267465-370142-267465WB3anti-fibromodulin-antibody-epr23029-174-western-blot-uterus-human.jpg "Anti-Fibromodulin antibody [EPR23029-174] (ab267465)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23029-174] to Fibromodulin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Fibromodulin antibody [EPR23029-174]
See all Fibromodulin primary antibodies -
Description
Rabbit monoclonal [EPR23029-174] to Fibromodulin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Recombinant Human Fibromodulin/FMOD protein, Human articular cartilage, Human uterus, Mouse articular cartilage, Rat articular cartilage, Human uterus and Human uterus treated with PNGase F lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23029-174 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Fibromodulin antibody [EPR23029-174] (ab267465)All lanes : Anti-Fibromodulin antibody [EPR23029-174] (ab267465) at 1/1000 dilution
Lane 1 : Human uterus tissue lysate
Lane 2 : Human uterus tissue treated with PNGase F lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 43 kDa
Observed band size: 60-100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 12623288, 8895216).
Two bands of 46kDa and 40kDa are de-glycosylated forms. The bands of 60-100kDa are glycosylated forms.
Exposure time: 3 minutes
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![Western blot - Anti-Fibromodulin antibody [EPR23029-174] (ab267465)](https://www.abcam.com/ps/products/267/ab267465/Images/ab267465-370141-267465WB2anti-fibromodulin-antibody-epr23029-174-western-blot.jpg)
Western blot - Anti-Fibromodulin antibody [EPR23029-174] (ab267465)All lanes : Anti-Fibromodulin antibody [EPR23029-174] (ab267465) at 1/1000 dilution
Lane 1 : Human articular cartilage lysate
Lane 2 : Human uterus lysate
Lane 3 : Human skin lysate
Lane 4 : HuT-78 (human sezary syndrome cutaneous t lymphocyte) whole cell lysate
Lane 5 : Mouse articular cartilage lysate
Lane 6 : Rat articular cartilage lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 5-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 43 kDa
Observed band size: 60-100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 12623288, 8895216)
Negative control: Hut-78 (PMID: 15741214)
Exposure time: Lane 1 second; Lanes 2-6:3 seconds
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![Western blot - Anti-Fibromodulin antibody [EPR23029-174] (ab267465)](https://www.abcam.com/ps/products/267/ab267465/Images/ab267465-370139-267465WB1anti-fibromodulin-antibody-epr23029-174-western-blot-recombinant-protein-human.jpg)
Western blot - Anti-Fibromodulin antibody [EPR23029-174] (ab267465)Anti-Fibromodulin antibody [EPR23029-174] (ab267465) at 1/1000 dilution + Recombinant Human Fibromodulin/FMOD protein (aa75-376) 20ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST Exposure time: 114 seconds
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![Anti-Fibromodulin antibody [EPR23029-174] (ab267465)](https://www.abcam.com/ps/products/267/ab267465/Images/ab267465-5-benefits-of-recombinant-antibodies.png)
Anti-Fibromodulin antibody [EPR23029-174] (ab267465)
