Anti-Histone H3 (symmetric di methyl R26) antibody (ab127095)
Key features and details
- Rabbit polyclonal to Histone H3 (symmetric di methyl R26)
- Suitable for: PepArr, ICC/IF, WB
- Reacts with: Cow, Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (symmetric di methyl R26) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (symmetric di methyl R26) -
Host species
Rabbit -
Tested applications
Suitable for: PepArr, ICC/IF, WBmore details -
Species reactivity
Reacts with: Cow, Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human Histone H3 aa 1-100 (symmetric di methyl R26) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab154427) -
Positive control
- WB: Calf thymus histone lysate. ICC/IF: formaldehyde fixed MCF-7 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Images
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Western blot - Anti-Histone H3 (symmetric di methyl R26) antibody (ab127095)Anti-Histone H3 (symmetric di methyl R26) antibody (ab127095) at 1 µg/ml + Calf Thymus Histone Preparation Nuclear Lysate at 0.25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutes
This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab127095 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution. -
Peptide Array - Anti-Histone H3 (symmetric di methyl R26) antibody (ab127095)All batches of ab127095 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - symmetric di methyl R26 peptide (ab154427), indicating that this antibody specifically recognises the Histone H3 - symmetric di methyl R26 modification.
- ab154427 - Histone H3 - symmetric di methyl R26
- ab2854 - Histone H3 - asymmetric di methyl R26
- ab154426 - Histone H3 - mono methyl R26
- ab17163 - Histone H3 - unmodified
- ab154424 - Histone H3 - mono methyl R17
- ab154425 - Histone H3 - symmetric di methyl R17
- ab16935 - Histone H3 - asymmetric di methyl R17
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (symmetric di methyl R26) antibody (ab127095)ICC/IF image of ab127095 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab127095 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
