Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Epigenetics and Nuclear Signaling Histones H3 Methylated

Alexa Fluor® 488 Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] (ab203850)

Price and availability

324 988 ₸

Availability

Order now and get it on Thursday February 25, 2021

Alexa Fluor® 488 Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] (ab203850)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Alexa Fluor® 488 Mouse monoclonal [mAbcam 1220] to Histone H3 (di methyl K9)
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Isotype: IgG2a

You may also be interested in

Histone H4 (mono-methyl K20) Quantification Kit (Fluorometric) (ab115094)
Product image
Anti-Histone H3 (symmetric di methyl R8) antibody (ab130740)
Product image
Alexa Fluor® 594 Anti-Histone H3 (di methyl K4) antibody [Y47] (ab207864)
Histone H3 (di-methyl K36) Quantification Kit (Fluorometric) (ab115078)

Overview

  • Product name

    Alexa Fluor® 488 Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220]
    See all Histone H3 primary antibodies
  • Description

    Alexa Fluor® 488 Mouse monoclonal [mAbcam 1220] to Histone H3 (di methyl K9)
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab1772)

  • Positive control

    • ICC/IF: HeLa cells.
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    mAbcam 1220
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Methylated

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] (ab203850)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] (ab203850)

    ab203850 staining Histone H3 (di methyl K9) in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203850 at a 1/100 dilution (shown in green) and ab202272, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Alexa Fluor® 488 Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] (ab203850)

  •  
  • Product image

    Anti-Histone H3 antibody [EPR16987] - ChIP Grade (ab176842)

    Applications: ChIP, Flow Cyt, ICC/IF, IHC-P, PepArr, WB

  •  
  • Product image

    Anti-Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade (ab52946)

    Applications: ChIP, ICC/IF, IHC-P, WB

  •  
  • Product image

    Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (ab219407)

    Applications: ELISA, Flow Cyt, ICC/IF, IP, WB

  •  
  • Product image

    Alexa Fluor® 488 Anti-Histone H3 (acetyl K14) antibody [EP964Y] (ab277918)

    Applications: ICC

  •  
  • Product image

    Alexa Fluor® 647 Anti-Histone H3 (acetyl K14) antibody [EP964Y] (ab277919)

    Applications: ICC

  •  
  • Product image

    Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916)

    Applications: ChIP, Dot, ICC/IF, IHC-P, PepArr, WB

  •  
  • Product image

    Anti-Histone H3 (tri methyl K27) antibody [EPR18607] - ChIP Grade (ab192985)

    Applications: ChIP, ChIP-seq, ELISA, ICC/IF, IHC-P, PepArr, WB

  •  
  • Product image

    Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (ab32356)

    Applications: ChIP, ChIP-seq, Flow Cyt, ICC/IF, IHC-P, IP, WB

Clear all

Recently viewed products

  •  
  • Product image

    Anti-ATG12 antibody [EPR4800] - BSA and Azide free (ab247876)

  •  
  • Product image

    FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)

  •  
  • Product image

    PE Anti-CD2 antibody [MEM-65] (ab269318)

  •  
  • Product image

    Anti-HCV subtype 1b NS5B antibody (ab35586)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.