Anti-PKC alpha + beta + gamma antibody [M499] (ab253274)
![Anti-PKC alpha + beta + gamma antibody [M499] (ab253274)](https://www.abcam.com/ps/products/253/ab253274/Images/ab253274-343739-AntiPKC-alpha--beta--gamma-antibody-M499-western-blot-adult-mouse-brain.jpg "Anti-PKC alpha + beta + gamma antibody [M499] (ab253274)")
Key features and details
- Mouse monoclonal [M499] to PKC alpha + beta + gamma
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG2a
Overview
-
Product name
Anti-PKC alpha + beta + gamma antibody [M499]
See all PKC alpha + beta + gamma primary antibodies -
Description
Mouse monoclonal [M499] to PKC alpha + beta + gamma -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Rabbit, Cow, Human, Cynomolgus monkey
-
Immunogen
Recombinant fragment corresponding to Human PKC alpha + beta + gamma aa 450 to the C-terminus. Relating to PKC gamma.
Database link: P05129 -
Positive control
- WB: Adult mouse brain lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
M499 -
Isotype
IgG2a -
Research areas
Images
-
Western blot - Anti-PKC alpha + beta + gamma antibody [M499] (ab253274)Lane 1 : Anti-PKC alpha + beta + gamma antibody [M499] (ab253274) at 1/250 dilution
Lane 2 : Anti-PKC alpha + beta + gamma antibody [M499] (ab253274) at 1/1000 dilution
All lanes : Adult mouse brain lysateWestern blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hr at RT.
This antibody detects 80-82kDa proteins corresponding to the molecular mass of PKC alpha, PKC beta and PKC gamma SDS-PAGE immunoblots of mouse brain lysates.
This antibody also detects recombinant PKC alpha, PKC beta 1, and PKC gamma, and possibly detects other PKC isoforms as well.