Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RIP3 (phospho S227) antibody [EPR9627] - BSA and Azide free (ab240383)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9627] to RIP3 (phospho S227) - BSA and Azide free
  • Suitable for: Dot blot, ELISA, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-RIP3 (phospho S227) antibody [EPR9627] - BSA and Azide free
    See all RIP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR9627] to RIP3 (phospho S227) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab240383 is the carrier-free version of ab209384. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240383 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RIP3 (phospho S227) antibody [EPR9627] - BSA and Azide free (ab240383)
    Western blot - Anti-RIP3 (phospho S227) antibody [EPR9627] - BSA and Azide free (ab240383)
    All lanes : Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384) at 1/1000 dilution

    Lane 1 : HT-29 (Human colorectal adenocarcinoma epithelial cell) treated with 20 ng/ml TNF alpha, 100 nM Smac mimetic and 20µM VAD-FMK for 6 h cell lysate
    Lane 2 : HT-29 (Human colorectal adenocarcinoma epithelial cell) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 57 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 100 seconds


    Blocking/Diluting buffer and concentration 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209384).

  • Dot Blot - Anti-RIP3 (phospho S227) antibody [EPR9627] - BSA and Azide free (ab240383)
    Dot Blot - Anti-RIP3 (phospho S227) antibody [EPR9627] - BSA and Azide free (ab240383)

    Dot blot analysis of RIP3 (phospho S227) phospho peptide (Lane 1) and RIP3 non-phospho peptide (Lane 2) labeled using ab209384 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209384).

  • ELISA - Anti-RIP3 (phospho S227) antibody [EPR9627] - BSA and Azide free (ab240383)
    ELISA - Anti-RIP3 (phospho S227) antibody [EPR9627] - BSA and Azide free (ab240383)

    Direct ELISA antibody dose-response curve using ab209384. Antigen concentration of 1000 ng/mL and primary antibody concentration of 0-1000 ng/mL. A Alkaline Phosphatase-conjugated AffiniPure -conjugated goat anti-rabbit IgG (1/2500) was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209384).

  • Anti-RIP3 (phospho S227) antibody [EPR9627] - BSA and Azide free (ab240383)
    Anti-RIP3 (phospho S227) antibody [EPR9627] - BSA and Azide free (ab240383)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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