Anti-RIP3 antibody (ab62344)
Key features and details
- Rabbit polyclonal to RIP3
- Suitable for: ICC/IF, IHC-FoFr, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-RIP3 antibody
See all RIP3 primary antibodies -
Description
Rabbit polyclonal to RIP3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatHumanIHC-P MouseWB MouseHuman
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Immunogen
Synthetic peptide corresponding to Mouse RIP3 (C terminal).
Database link: Q9Z2P5 -
Positive control
- WB: Jurkat, A431, HepG2 and C2C12 cell lysates. IF/ICC: SV40LT-SMC cell line IHC-P: Mouse kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RIP3 antibody (ab62344)All lanes : Anti-RIP3 antibody (ab62344) at 0.5 µg/ml
Lane 1 : A431 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 57 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIP3 antibody (ab62344)Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with ab62344 at 2.5 μg/mL. Sections were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Western blot - Anti-RIP3 antibody (ab62344)Lanes 1-2 : Anti-RIP3 antibody (ab62344) at 0.1 µg/ml
Lane 3 : Anti-RIP3 antibody (ab62344) at 0.2 µg/ml
Lane 4 : Anti-RIP3 antibody (ab62344) at 0.5 µg/ml
Lane 1 : C2C12 (mouse myoblast cell line) cell lysate with Peptide blocking
Lanes 2-4 : C2C12 (mouse myoblast cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 57 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-RIP3 antibody (ab62344)Immunofluorescence of RIP3 in rat Kidney cells using ab62344 at 20 ug/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-RIP3 antibody (ab62344)ICC/IF image of ab62344 stained SV40LT-SMC cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62344, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
