Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BPR antibody [EPR5201] - BSA and Azide free (ab247638)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5201] to BPR - BSA and Azide free
  • Suitable for: ICC, IHC-P, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-BPR antibody [EPR5201] - BSA and Azide free
    See all BPR primary antibodies

  • Description

    Rabbit monoclonal [EPR5201] to BPR - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247638 is the carrier-free version of ab108346 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247638 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as BCL2L12

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-BPR antibody [EPR5201] - BSA and Azide free (ab247638)
    Western blot - Anti-BPR antibody [EPR5201] - BSA and Azide free (ab247638)
    All lanes : Anti-BPR antibody [EPR5201] (ab108346) at 1/1000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : HT-1376 cell lysate
    Lane 3 : MCF-7 cell lysate
    Lane 4 : PC-3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 37 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab108346, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-BPR antibody [EPR5201] - BSA and Azide free (ab247638)
    Immunocytochemistry - Anti-BPR antibody [EPR5201] - BSA and Azide free (ab247638)
    This data was developed using ab108346, the same antibody clone in a different buffer formulation.ICC/IF image of ab108346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108346, 1/100) overnight at +4°C. The secondary antibody (green) was ab96899, a goat anti-rabbit DyLight® 488 (IgG; H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
  • Flow Cytometry - Anti-BPR antibody [EPR5201] - BSA and Azide free (ab247638)
    Flow Cytometry - Anti-BPR antibody [EPR5201] - BSA and Azide free (ab247638)
    This data was developed using ab108346, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab108346 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108346, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit Alexa Fluor® 488 (IgG; H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BPR antibody [EPR5201] - BSA and Azide free (ab247638)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BPR antibody [EPR5201] - BSA and Azide free (ab247638)
    This data was developed using ab108346, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of BPR in paraffin embedded Human breast carcinoma tissue, using ab108346 at a dilution of 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-BPR antibody [EPR5201] - BSA and Azide free (ab247638)
    Anti-BPR antibody [EPR5201] - BSA and Azide free (ab247638)

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