HRP Anti-KAT3B / p300 antibody [3G230 / NM-11] (ab200186)
![HRP Anti-KAT3B / p300 antibody [3G230 / NM-11] (ab200186)](https://www.abcam.com/ps/products/200/ab200186/Images/ab200186-246657-anti-kat3b-p300-antibody-3g230-nm-11-hrp-western-blot.jpg "HRP Anti-KAT3B / p300 antibody [3G230 / NM-11] (ab200186)")
Key features and details
- HRP Mouse monoclonal [3G230 / NM-11] to KAT3B / p300
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-KAT3B / p300 antibody [3G230 / NM-11]
See all KAT3B / p300 primary antibodies -
Description
HRP Mouse monoclonal [3G230 / NM-11] to KAT3B / p300 -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant full length protein corresponding to Human KAT3B/ p300.
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Positive control
- WB: Jurkat whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3G230 / NM-11 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - HRP Anti-KAT3B / p300 antibody [3G230 / NM-11] (ab200186)HRP Anti-KAT3B / p300 antibody [3G230 / NM-11] (ab200186) at 1/5000 dilution + Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 300 kDa
Observed band size: 300,75 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab200186 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
