Alexa Fluor® 488 Anti-hnRNP K antibody [F45 P9 C7] (ab204249)
![Alexa Fluor® 488 Anti-hnRNP K antibody [F45 P9 C7] (ab204249)](https://www.abcam.com/ps/products/204/ab204249/Images/ab204249-248161-anti-hnrnp-k-antibody-f45-p9-c7-alexa-fluor-488-immunofluorescence.jpg "Alexa Fluor® 488 Anti-hnRNP K antibody [F45 P9 C7] (ab204249)")
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [F45 P9 C7] to hnRNP K
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG1
Overview
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Product name
Alexa Fluor® 488 Anti-hnRNP K antibody [F45 P9 C7]
See all hnRNP K primary antibodies -
Description
Alexa Fluor® 488 Mouse monoclonal [F45 P9 C7] to hnRNP K -
Host species
Mouse -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
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Positive control
- ICC/IF: HeLa cells.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
F45 P9 C7 -
Myeloma
Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-hnRNP K antibody [F45 P9 C7] (ab204249)
ab204249 staining hnRNP K in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204249 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).
