Anti-srGAP2 antibody (ab121977)
Key features and details
- Rabbit polyclonal to srGAP2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG
Overview
-
Product name
Anti-srGAP2 antibody
See all srGAP2 primary antibodies -
Description
Rabbit polyclonal to srGAP2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human, African green monkey -
Immunogen
antigen: LEPLKTSPVV APTSEPSSPL HTQLLKDPEP AFQRSASTAG DIACAFRPVK SVKMAAPVKP PATRPKPTVF PKTNATSPGV NSSTSPQST, corresponding to amino acids 977-1065 of Human srGAP2.
-
General notes
ab121977 is mono-specific.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
ab121977 is mono-specific. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-srGAP2 antibody (ab121977) This image is courtesy of Alain Chedotal (Institut Vision, France)All lanes : Anti-srGAP2 antibody (ab121977) at 1/100 dilution
Lane 1 : COS7 Whole cell lysate
Lane 2 : HEK293H Whole Cell Lysate
Lane 3 : HEK293H Over expressing human srGAP1
Lane 4 : HEK293H Over expressing human srGAP2
Lane 5 : HEK293H Over expressing human srGAP3
Lane 6 : HeLa cells over expressing mouse srGAP2
Lane 7 : Mouse E14 hindbrain lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG Conjugated to HRP at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 121 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa (possible tagged protein)
Exposure time: 5 minutesBlocking: 5% MIlk
-
Immunocytochemistry/ Immunofluorescence - Anti-srGAP2 antibody (ab121977)Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121977 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-srGAP2 antibody (ab121977)ab121977, at 1/10 dilution, staining srGAP2 in paraffin-embedded Human adrenal gland tissue by Immunohistochemistry.
