Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

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Anti-ASH2L antibody [EPR13106] (ab181117)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13106] to ASH2L
Suitable for: WB, ICC/IF, Flow Cyt, IP
Reacts with: Rat, Human

Product name

Anti-ASH2L antibody [EPR13106]
See all ASH2L primary antibodies
Description

Rabbit monoclonal [EPR13106] to ASH2L
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa cells, 293T cell line lysate, K562 cell line lysate, C6 cell line lysate, PC12 cell line lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13106
Isotype

IgG
Research areas

Western blot - Anti-ASH2L antibody [EPR13106] (ab181117)

All lanes : Anti-ASH2L antibody [EPR13106] (ab181117) at 1/10000 dilution

Lane 1 : 293T cell line lysate
Lane 2 : K562 cell line lysate
Lane 3 : C6 cell line lysate
Lane 4 : PC12 cell line lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 69 kDa
Observed band size: 80 kDa
why is the actual band size different from the predicted?
Flow Cytometry - Anti-ASH2L antibody [EPR13106] (ab181117)

Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling ASH2L with ab181117 at 1/20 followed by Goat anti rabbit IgG (FITC) at 1/150 (pink). Rabbit monoclonal IgG was used as Isotype control (green).
Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13106] (ab181117)

Immunofluorescent analysis of acetone fixed HeLa cells labeling ASH2L with ab181117 at 1/100 (green). Staining with Dapi is shown in blue.
Immunoprecipitation - Anti-ASH2L antibody [EPR13106] (ab181117)

Immunoprecipitation analysis of 293T cells labeling ASH2L using ab181117 at 1/60 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 was used as secondary antibody.
Anti-ASH2L antibody [EPR13106] (ab181117)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com