Rabbit monoclonal [RM217] Anti-Human IgG4 Fc (Biotin) (ab272185)
![Rabbit monoclonal [RM217] Anti-Human IgG4 Fc (Biotin) (ab272185)](https://www.abcam.com/ps/products/272/ab272185/Images/ab272185-385422-biotin-antihuman-IgG4-Fc-antibody-rm217-elisa-specificity.JPG "Rabbit monoclonal [RM217] Anti-Human IgG4 Fc (Biotin) (ab272185)")
Key features and details
- Rabbit monoclonal [RM217] Anti-Human IgG4 Fc (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG4
- Suitable for: ELISA
Overview
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Product name
Rabbit monoclonal [RM217] Anti-Human IgG4 Fc (Biotin)
See all IgG4 secondary antibodies -
Host species
Rabbit -
Target species
Human -
Tested applications
Suitable for: ELISAmore details -
Immunogen
Synthetic peptide corresponding to Human IgG4. Peptide corresponding to the Fc region of Human IgG4.
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
From an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM217 -
Isotype
IgG4 -
Research areas
Images
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ELISA - Rabbit monoclonal [RM217] Anti-Human IgG4 Fc (Biotin) (ab272185)
ELISA shows that ab272185 specifically reacts to human IgG4 and does not cross react with human IgG1, IgG2, IgG3.
ab272185 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
50ng/well to 200ng/well was used for capture, and 0.05ug/mL to 0.2ug/mL was used for detection.
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ELISA - Rabbit monoclonal RM217 Anti-Human IgG4 Fc (Biotin) (ab272185)A titer ELISA analysis using ab272185. The plate was coated with different amounts of human IgG4. A serial dilution of ab272185 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Sandwich ELISA - Rabbit monoclonal RM217 Anti-Human IgG4 Fc (Biotin) (ab272185)Sandwich ELISA using ab272185 as the capture antibody, and a Biotinylated anti-human light chains (κ+ λ) antibody ab193185 as the detection antibody, followed by an AP conjugated streptavidin.
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Sandwich ELISA - Rabbit monoclonal RM217 Anti-Human IgG4 Fc (Biotin) (ab272185)Sandwich ELISA using ab272185 as the capture antibody, and a Biotinylated anti-human light chains (κ+ λ) antibody ab193185 as the detection antibody, followed by an AP conjugated streptavidin.