Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKC beta 2 antibody [Y125] (ab32026)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y125] to PKC beta 2
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-PKC beta 2 antibody [Y125]
    See all PKC beta 2 primary antibodies

  • Description

    Rabbit monoclonal [Y125] to PKC beta 2

  • Host species

    Rabbit

  • Specificity

    ab32026 recognises PKC beta 2, it does not cross react with human PKC beta 1. Immunogen's homology with PKC alpha is 77.8%. Cross-reactivity with PKC alpha has not been tested.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Rat
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PKC beta 2 aa 600-700 (C terminal). The exact sequence is proprietary.

  • Epitope

    ab32026 reacts with an epitope located in the region near the C terminus of PKC beta 2.

  • Positive control

    • K562 cell lysate, HeLa cell lysate, rat spinal cord lysate and human breast carcinoma

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    Y125

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PKC beta 2 antibody [Y125] (ab32026)
    Western blot - Anti-PKC beta 2 antibody [Y125] (ab32026)
    Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/500 dilution + Recombinant human PKC beta 2 protein (ab60841) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 77 kDa
    Observed band size: 103 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 seconds


    Blocking Buffer: 5% NFDM/TBST

    Diluting Buffer: 5% NFDM/TBST
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC beta 2 antibody [Y125] (ab32026)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC beta 2 antibody [Y125] (ab32026)

    ab32026, at a 1/100 dilution, staining human PKC beta 2 in breast carcinoma by Immunohistochemistry, Paraffin embedded tissue

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-PKC beta 2 antibody [Y125] (ab32026)
    Immunocytochemistry/ Immunofluorescence - Anti-PKC beta 2 antibody [Y125] (ab32026)
    ab32026, at a 1/250 dilution, staining human PKC beta 2 in HeLa cells by Immunoflourescence
  • Flow Cytometry - Anti-PKC beta 2 antibody [Y125] (ab32026)
    Flow Cytometry - Anti-PKC beta 2 antibody [Y125] (ab32026)

    Overlay histogram showing HeLa cells stained with ab32026 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32026, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-PKC beta 2 antibody [Y125] (ab32026)
    Western blot - Anti-PKC beta 2 antibody [Y125] (ab32026)
    All lanes : Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/500 dilution

    Lane 1 : K562 whole cell lysates at 20 µg
    Lane 2 : Active human PKC beta 2 full length protein at 0.02 µg
    Lane 3 : Human fetal brain at 20 µg
    Lane 4 : NIH/3T3 whole cell lysates at 20 µg
    Lane 5 : Raw264.7 whole cell lysates at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 77 kDa
    Observed band size: 77 kDa


    Exposure time: 15 seconds


    Blocking buffer: 5% NFDM/TBST

    Diluting buffer: 5% NFDM/TBST

    The PKC beta 2 recombinant protein is GST-tagged, so the molecular weight is higher.

  • Western blot - Anti-PKC beta 2 antibody [Y125] (ab32026)
    Western blot - Anti-PKC beta 2 antibody [Y125] (ab32026)
    Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/500 dilution + Rat spinal cord at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 77 kDa
    Observed band size: 77 kDa


    Exposure time: 3 minutes


    Blocking Buffer: 5% NFDM/TBST

    Diluting Buffer: 5% NFDM/TBST

  • Western blot - Anti-PKC beta 2 antibody [Y125] (ab32026)
    Western blot - Anti-PKC beta 2 antibody [Y125] (ab32026)
    Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/500 dilution + K562 cell lysate

    Predicted band size: 77 kDa
    Observed band size: 77 kDa

  • Western blot - Anti-PKC beta 2 antibody [Y125] (ab32026)
    Western blot - Anti-PKC beta 2 antibody [Y125] (ab32026)
    All lanes : Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/1000 dilution

    Lane 1 : MCF-7 whole cell lysates
    Lane 2 : HeLa whole cell lysates
    Lane 3 : K562 whole cell lysates
    Lane 4 : Recombinant human PKC alpha protein (Active) (ab55672)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 77 kDa
    Observed band size: 77 kDa


    Exposure time: 30 seconds


    Blocking Buffer: 5% NFDM/TBST

    Diluting Buffer: 5% NFDM/TBST
  • Anti-PKC beta 2 antibody [Y125] (ab32026)
    Anti-PKC beta 2 antibody [Y125] (ab32026)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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