Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (ab250576)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17074] to PKC delta + PKC theta - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free
    See all PKC delta + PKC theta primary antibodies

  • Description

    Rabbit monoclonal [EPR17074] to PKC delta + PKC theta - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250576 is the carrier-free version of ab182127. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250576 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17074

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (ab250576)
    Western blot - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (ab250576)
    All lanes : Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127) at 1/2000 dilution

    Lane 1 : Active human PKC alpha full length protein
    Lane 2 : Active human PKC beta 1 full length protein
    Lane 3 : Active human PKC beta 2 full length protein
    Lane 4 : Active human PKC gamma full length protein
    Lane 5 : Active human PKC delta full length protein
    Lane 6 : Active human PKC eta full length protein
    Lane 7 : Active human PKC epsilon full length protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 78, 82 kDa


    Exposure time: 5 seconds


    This data was developed using ab182127, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Active human PKC alpha full length protein (ab55672) contains aa1-672 with GST-tag; Active human PKC beta 1 full length protein (ab60840) contains aa1-671 with GST-tag; Active human PKC beta 2 full length protein (ab60841) contains aa1-673 with GST-tag; Active human PKC gamma full length protein (ab60842) contains aa1-677 with GST-tag; Active human PKC delta full length protein (ab60844) contains aa1-676 with GST-tag; Active human PKC eta full length protein (ab60849) contains aa1-683 with GST-tag; Active human PKC epsilon full length protein (ab60847) contains aa1-737 with GST-tag; Active human PKC theta full length protein (ab56641) contains aa1-706 with GST-tag; Active human PKC mu full length protein (ab60873) contains aa1-912 with GST-tag.

  • Immunocytochemistry - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (ab250576)
    Immunocytochemistry - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (ab250576)

    This data was developed using ab182127, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling PKC delta + PKC theta with ab182127 at 1/200 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing both cytoplasm and nuclear staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    1. ab182127 at 1/200 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (ab250576)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (ab250576)

    This data was developed using ab182127, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PKC delta + PKC theta with ab182127 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on lymphocytes on germinal center of Human tonsil is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (ab250576)
    Western blot - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (ab250576)
    All lanes : Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127) at 1/2000 dilution

    Lane 1 : Mouse thymus lysates
    Lane 2 : Rat thymus lysates
    Lane 3 : Molt4 (Human lymphoblastic leukemia cell line) whole cell lysates
    Lane 4 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 78, 82 kDa
    Observed band size: 78 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab182127, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (ab250576)
    Western blot - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (ab250576)
    Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127) at 1/2000 dilution + Human fetal brain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 78, 82 kDa
    Observed band size: 78 kDa why is the actual band size different from the predicted?



    This data was developed using ab182127, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (ab250576)
    Western blot - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (ab250576)
    All lanes : Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127) at 1/10000 dilution

    Lane 2 : Rat brain tissue lysate
    Lane 3 : Mouse brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 78, 82 kDa
    Observed band size: 78 kDa why is the actual band size different from the predicted?



    This data was developed using ab182127, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (ab250576)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (ab250576)
    This data was developed using ab182127, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling PKC delta + PKC theta with ab182127 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on cancer cells of Human breast carcinoma is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (ab250576)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (ab250576)
    This data was developed using ab182127, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse kidney cancer tissue labeling PKC delta + PKC theta with ab182127 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on mouse kidney tubules is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (ab250576)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (ab250576)
    This data was developed using ab182127, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat spleen cancer tissue labeling PKC delta + PKC theta with ab182127 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on monocytes and macrophages on rat spleen is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (ab250576)
    Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (ab250576)

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