Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKC antibody [EPR16791] (ab179523)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16791] to PKC
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-PKC antibody [EPR16791]
    See all PKC primary antibodies

  • Description

    Rabbit monoclonal [EPR16791] to PKC

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Active human PKC alpha, PKC beta 1, PKC beta 2, PKC gamma, PKC delta, PKC eta, PKC epsilon and PKC theta full length proteins; Human fetal heart and skin lysates; Mouse lung, heart, kidney and spleen lysates; MCF7 and PC-3 whole cell lysates; Rat heart and spleen lysates. IP: Mouse lung whole cell lysate.

  • General notes

    Recombinant PKC isoforms were tested for reactivity to ab179521 by western blot, and are marked as positive (+) or negative (-).

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16791

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PKC antibody [EPR16791] (ab179523)
    Western blot - Anti-PKC antibody [EPR16791] (ab179523)
    All lanes : Anti-PKC antibody [EPR16791] (ab179523) at 1/2000 dilution

    Lane 1 : Active human PKC alpha full length protein
    Lane 2 : Active human PKC beta 1 full length protein
    Lane 3 : Active human PKC beta 2 full length protein
    Lane 4 : Active human PKC gamma full length protein
    Lane 5 : Active human PKC delta full length protein
    Lane 6 : Active human PKC mu full length protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Active human PKC alpha full length protein (Catalog#ab55672) contains aa1-672 with GST-tag; Active human PKC beta 1 full length protein (Catalog#ab60840) contains aa1-671 with GST-tag; Active human PKC beta 2 full length protein (Catalog#ab60841) contains aa1-673 with GST-tag; Active human PKC gamma full length protein (Catalog#ab60842) contains aa1-677 with GST-tag; Active human PKC delta full length protein (Catalog#ab60844) contains aa1-676 with GST-tag; Active human PKC mu full length protein (Catalog#ab60873) contains aa1-912 with GST-tag.

  • Western blot - Anti-PKC antibody [EPR16791] (ab179523)
    Western blot - Anti-PKC antibody [EPR16791] (ab179523)
    All lanes : Anti-PKC antibody [EPR16791] (ab179523) at 1/2000 dilution

    Lane 1 : Active human PKC eta full length protein
    Lane 2 : Active human PKC epsilon full length protein
    Lane 3 : Active human PKC theta full length protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Active human PKC eta full length protein (Catalog#ab60849) contains aa1-683 with GST-tag; Active human PKC epsilon full length protein (Catalog#ab60847) contains aa1-737 with GST-tag; Active human PKC theta full length protein (Catalog#ab56641) contains aa1-706 with GST-tag.

  • Western blot - Anti-PKC antibody [EPR16791] (ab179523)
    Western blot - Anti-PKC antibody [EPR16791] (ab179523)
    All lanes : Anti-PKC antibody [EPR16791] (ab179523) at 1/1000 dilution

    Lane 1 : Active human PKC zeta full length protein
    Lane 2 : Active human PKC iota full length protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 78 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Active human PKC zeta full length protein (Catalog# ab60848) contains aa1-592 with GST-tag; Active human PKC iota full length protein (Catalog#ab60850) contains aa1-596 with GST-tag.

  • Western blot - Anti-PKC antibody [EPR16791] (ab179523)
    Western blot - Anti-PKC antibody [EPR16791] (ab179523)

    Recombinant PKC isoforms were tested for reactivity by western blot, and are marked as positive (+) or negative (-).

  • Western blot - Anti-PKC antibody [EPR16791] (ab179523)
    Western blot - Anti-PKC antibody [EPR16791] (ab179523)
    Anti-PKC antibody [EPR16791] (ab179523) at 1/2000 dilution + Human fetal heart lysate at 1/10 dilution

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 78 kDa
    Observed band size: 78 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Western blot - Anti-PKC antibody [EPR16791] (ab179523)
    Western blot - Anti-PKC antibody [EPR16791] (ab179523)
    All lanes : Anti-PKC antibody [EPR16791] (ab179523) at 1/10000 dilution

    Lane 1 : Human fetal skin lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 3 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
    Lane 4 : Mouse lung lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa
    Observed band size: 40,78 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKCs (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331.

    Newton AC, 1995. J. Biol. Chem. 270, 28495-28498).

  • Western blot - Anti-PKC antibody [EPR16791] (ab179523)
    Western blot - Anti-PKC antibody [EPR16791] (ab179523)
    All lanes : Anti-PKC antibody [EPR16791] (ab179523) at 1/2000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat heart lysate
    Lane 5 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa
    Observed band size: 40,78 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKCs (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331.

    Newton AC, 1995. J. Biol. Chem. 270, 28495-28498).

  • Immunoprecipitation - Anti-PKC antibody [EPR16791] (ab179523)
    Immunoprecipitation - Anti-PKC antibody [EPR16791] (ab179523)

    PKC was immunoprecipitated from 1 mg of Mouse lung whole cell lysate with ab179523 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab179523 at 1/1000 dilution.

    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Mouse lung whole cell lysate, 10µg (Input).

    Lane 2: ab179523 IP in Mouse lung whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal[EPR25A]-Isotype Control (ab172730) instead of ab179523 in Mouse lung whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-PKC antibody [EPR16791] (ab179523)
    Anti-PKC antibody [EPR16791] (ab179523)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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