Anti-PKC antibody (ab19031)
Key features and details
- Rabbit polyclonal to PKC
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-PKC antibody
See all PKC primary antibodies -
Description
Rabbit polyclonal to PKC -
Host species
Rabbit -
Specificity
ab19031 recognizes the alpha, beta and gamma isoforms of PKC. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Rat
-
Immunogen
Synthetic peptide corresponding to Mouse PKC (C terminal). Synthetic peptide corresponding to the C-terminus of mouse PKC ß1
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-PKC antibody (ab19031)Anti-PKC antibody (ab19031) at 0.5 µg/ml + Cell lysate prepared from rat kidney cells
Predicted band size: 82 kDa
-
Immunocytochemistry/ Immunofluorescence - Anti-PKC antibody (ab19031)ICC/IF image of ab19031 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19031, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
