Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] - BSA and Azide free (ab249448)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8269(2)] to NEDD4-2 (phospho S448) - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] - BSA and Azide free
See all NEDD4-2 primary antibodies -
Description
Rabbit monoclonal [EPR8269(2)] to NEDD4-2 (phospho S448) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human NEDD4-2 (phospho S448). The exact sequence is proprietary. Corresponding to residues surrounding Serine 448.
Database link: Q96PU5 -
General notes
ab249448 is the carrier-free version of ab168349.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR8269(2) -
Isotype
IgG -
Research areas
Images
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This data was developed using ab168349, the same antibody clone in a different buffer formulation.Detection of NEDD4-2 by Western Blot of Immunprecipitate.
293T cell lysate immunoprecipitated using ab168349 at 1/10 dilution. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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All lanes : Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349) at 1/1000 dilution
Lane 1 : 293T cell lysate, untreated
Lane 2 : 293T cell lysate, treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 112 kDa
Additional bands at: 110-135 kDa. We are unsure as to the identity of these extra bands.This data was developed using ab168349, the same antibody clone in a different buffer formulation.
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