Anti-PDE6D antibody (ab96825)
Key features and details
- Rabbit polyclonal to PDE6D
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PDE6D antibody
See all PDE6D primary antibodies -
Description
Rabbit polyclonal to PDE6D -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Zebrafish
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Immunogen
Recombinant fragment corresponding to Human PDE6D aa 1-120.
Database link: NP_002592 -
Positive control
- WB: MOLT4 whole cell lysate; mouse eye lysate. IHC-P: SAS xenograft tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6D antibody (ab96825)
Paraffin-embedded SAS xenograft tissue labeling PDE6D with ab96825 at 1/500 dilution in immunohistochemical analysis.
Antigen retrieval: EDTA-based (pH 8.0), 15 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-PDE6D antibody (ab96825)Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling PDE6D with ab96825 at 1/500 dilution (green) in ICC/IF.
The nuclear counter stain is Hoechst 33342 (blue).
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Western blot - Anti-PDE6D antibody (ab96825)Anti-PDE6D antibody (ab96825) at 1/5000 dilution + Mouse eye lysate at 50 µg
Secondary
Anti-Rabbit IgG HRP
Predicted band size: 17 kDa
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Western blot - Anti-PDE6D antibody (ab96825)Anti-PDE6D antibody (ab96825) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 17 kDa
12% SDS PAGE
