Anti-Epac1 antibody (ab21236)
Key features and details
- Rabbit polyclonal to Epac1
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Epac1 antibody
See all Epac1 primary antibodies -
Description
Rabbit polyclonal to Epac1 -
Host species
Rabbit -
Specificity
ab21236 does not cross react with Epac2. It has not been tested against endogenous Epac1 but recombinant Epac1. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide:
DHSVLTLQLPVTASVR
, corresponding to amino acids 526-541 of Epac1. -
General notes
Ab21236 is supplied in an antibody stabilisation buffer. IgG concentration 0.78-0.94mg/ml in antibody stabilisation buffer.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Epac1 antibody (ab21236)ICC/IF image of ab21236 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21236, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
