Recombinant human PDE4A protein (ab198423)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human PDE4A protein
See all PDE4A proteins and peptides -
Biological activity
Specific Activity: ≥ 11,610 pmol/min/µg
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Assayed using a PDE4A Assay Kit. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198423 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
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Purity
40 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
EPPTVPSERSLSLSLPGPREGQATLKPPPQHLWRQPRTPIRIQQRGYSDS AERAERERQPHRPIERADAMDTSDRPGLRTTRMSWPSSFHGTGTGSGGAG GGSSRRFEAENGPTPSPGRSPLDSQASPGLVLHAGAATSQRRESFLYRSD SDYDMSPKTMSRNSSVTSEAHAEDLIVTPFAQVLASLRSVRSNFSLLTNV PVPSNKRSPLGGPTPVCKATLSEETCQQLARETLEELDWCLEQLETMQTY RSVSEMASHKFKRMLNRELTHLSEMSRSGNQVSEYISTTFLDKQNEVEIP SPTMKEREKQQAPRPRPSQPPPPPVPHLQPMSQITGLKKLMHSNSLNNSN IPRFGVKTDQEELLAQELENLNKWGLNIFCVSDYAGGRSLTCIMYMIFQE RDLLKKFRIPVDTMVTYMLTLEDHYHADVAYHNSLHAADVLQSTHVLLAT PALDAVFTDLEILAALFAAAIHDVDHPGVSNQFLINTNSELALMYNDESV LENHHLAVGFKLLQEDNCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMT LLADLKTMVETKKVTSSGVLLLDNYSDRIQVLRNMVHCADLSNPTKPLEL YRQWTDRIMAEFFQQGDRERERGMEISPMCDKHTASVEKSQVGFIDYIVH PLWETWADLVHPDAQEILDTLEDNRDWYYSAIRQSPSPPPEEESRGPGHP PLPDKFQFELTLEEEEEEEISMAQIPCTAQEALTAQGLSGVEEALDATIA WEASPAQESLEVMAQEASLEAELEAVYLTQQAQSTGSAPVAPDEFSSREE FVVAVSHSSPSALALQSPLLPAWRTLSVSEHAPGLPGLPSTAAEVEAQRE HQAAKRACSACAGTFGEDTSALPAPGGGGSGGDPT -
Predicted molecular weight
124 kDa including tags -
Amino acids
2 to 886 -
Tags
GST tag N-Terminus -
Additional sequence information
NM_001111307.
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Preparation and Storage
- 5''-cyclic phosphodiesterase 4A
- cAMP specific phosphodiesterase
- cAMP-specific 3''
Images
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SDS-PAGE - Recombinant human PDE4A protein (ab198423)
10% SDS-PAGE analysis of ab198423 (10 μg).
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Functional Studies - Recombinant human PDE4A protein (ab198423)Activity assay using ab198423.
