Recombinant rat PDE2A protein (ab198484)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 56% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant rat PDE2A protein
See all PDE2A proteins and peptides -
Biological activity
Specific Activity: ≥ 2500 pmol/min/µg
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198484 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. -
Purity
>= 56 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Rat -
Sequence
MHHHHHHGGGSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKW RNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAE ISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKT YLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDK YLKSSKYIAWPLQGWQATFGGGDHPPKSDPLVPRGSPGEF GQACGHSI LCRSQQYPAARPAEPRGQQVFLKPDEPPPQPCADSLQDALLSL GAVID IAGLRQAAKDALSAVLPKVETVYTYLVDGESRLVCEDPPHELPQEGKIRE AVISRKRLSCDGLGPSDLLGKPLARLVAPLAPDTQVLVIPLLDKETGTVA AVILVHCGQLSDSEEQSLQVVEKHALVALQRVQALQQRRPEAVQNTSADP SEDQKDEKGYTAHDRKILQLCGELYDLDATSLQLKVLRYLQQETQATHCC LLLVSEDNLQLSCKVIGEKVLGEEVSFPLTMGRLGQVVEDKQCIQLKDLT SDDVQQLQNMLGCELRAMLCVPVISRATDQVVALACAFNKLGGDFFTDED ERAIQHCFHYTGTVLTSTLAFQKEQKLKCECQALLQVAKNL FTHLDDV SVLLQEIITEARNLSNAEICSVFLLDQNELVAKVFDGGVVDDESYEIRIP ADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRNILCFPIKNE NQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEA QYRSHLANEMMMYHMKVSDDEYTKLLHDGIQPVAAIDSNFANFTYTPRSL PEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYRDPPYHNWMH AFSVSHFCYLLYKNLELSNYLEDIEIFALFISCMCHDLDHRGTNNSFQVA SKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSRKDYQR ML DLMRDIILATDLAHHLRIFKDLQKMAEVGYDRNNKQHHRLLLCLLMTSCD LSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDREKAYIPEL QISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLP SNNSLDFLDEEYEVPDLDVTRAPVNGCCSLEG -
Predicted molecular weight
133 kDa including tags -
Amino acids
18 to 928 -
Tags
His tag N-Terminus , GST tag N-Terminus -
Additional sequence information
His-GST tag and rat PDE2A protein are connected by a linker (PLVPRGSPGEF) including Thrombin Cleavage Site. NM_001143847
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine), 0.12% GlutathioneThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant rat PDE2A protein (ab198484)
Example of specific activity of ab198484
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SDS-PAGE - Recombinant rat PDE2A protein (ab198484)10% SDS PAGE analysis of ab198484 (5 μg).
