Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant human EPO protein (Fc Chimera Active) (ab243758)

Key features and details

  • Expression system: Mammalian
  • Endotoxin level:
  • Active: Yes
  • Tags: Fc tag C-Terminus
  • Suitable for: Functional Studies, HPLC, SDS-PAGE

Description

  • Product name

    Recombinant human EPO protein (Fc Chimera Active)
    See all EPO proteins and peptides

  • Biological activity

    Fully biologically active when compared to standard. The specific activity was measured by the stimulation of reticulocyte production in normocyth-aemic mice and is no less than 5.0 × 10^5 IU/mg.

  • Endotoxin level

  • Expression system

    Mammalian

  • Accession

    P01588

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      APPRLICDSRVLERYLLEAKEAENITTGCAEHCSLNENITVPDTKVNFYA WKRMEVGQQAVEVWQGLALLSEAVLRGQALLVNSSQPWEPLQLHVDKAVS GLRSLTTLLRALGAQKEAISPPDAASAAPLRTITADTFRKLFRVYSNFLR GKLKLYTGEACRTGDR+IEGRMDEPKSSDKTHTCPPCPAPEFEGAPSVFL FPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPR EEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPTPIEKTISKAKGQ PREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYK TTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLS LSPGK

    • Predicted molecular weight

      45 kDa including tags

    • Amino acids

      28 to 193

    • Tags

      Fc tag C-Terminus

    • Additional sequence information

      Full length mature chain without signal peptide.

    • Associated products

      Specifications

      Our Abpromise guarantee covers the use of ab243758 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        Functional Studies

        HPLC

        SDS-PAGE

      • Form

        Lyophilized
      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

        Constituent: PBS

        0.2 µm filtered

        This product is an active protein and may elicit a biological response in vivo, handle with caution.

      • Reconstitution
        We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at = -20degC. Further dilutions should be made in appropriate buffered solutions.

      General Info

      • Alternative names

        • EP
        • EPO
        • EPO alpha
        • Epoetin
        • Erythropoetin
        • Erythropoietin precursor
        • MVCD2
        see all

      • Relevance

        Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

      • Cellular localization

        Secreted

    Images

    • SDS-PAGE - Recombinant human EPO protein (Fc Chimera Active) (ab243758)
      SDS-PAGE - Recombinant human EPO protein (Fc Chimera Active) (ab243758)
      SDS Page analysis of EPO with ab243758.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab243758? Please let us know so that we can cite the reference in this datasheet.

    ab243758 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Alternative names

      • EP
      • EPO
      • EPO alpha
      • Epoetin
      • Erythropoetin
      • Erythropoietin precursor
      • MVCD2
      see all

    • Relevance

      Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

    • Cellular localization

      Secreted

    Images

    • SDS-PAGE - Recombinant human EPO protein (Fc Chimera Active) (ab243758)
      SDS-PAGE - Recombinant human EPO protein (Fc Chimera Active) (ab243758)
      SDS Page analysis of EPO with ab243758.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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