Anti-ATP6V1H antibody (ab254685)
Key features and details
- Rabbit polyclonal to ATP6V1H
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATP6V1H antibody
See all ATP6V1H primary antibodies -
Description
Rabbit polyclonal to ATP6V1H -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Pig
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Immunogen
Recombinant fragment corresponding to Human ATP6V1H aa 400 to the C-terminus.
Database link: Q9UI12 -
Positive control
- IHC-P: Human cerebral cortex, prostate, kidney and fallopian tube tissue. ICC/IF: U-2 OS cells. WB: HEK-293T overexpressing ATP6V1H with a C-terminal myc-DDK tag, cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1H antibody (ab254685)PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling ATP6V1H using ab254685 at 4µg/ml (green) in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1H antibody (ab254685)Paraffin-embedded human fallopian tube tissue stained for ATP6V1H using ab254685 at 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1H antibody (ab254685)Paraffin-embedded human kidney tissue stained for ATP6V1H using ab254685 at 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1H antibody (ab254685)Paraffin-embedded human prostate tissue stained for ATP6V1H using ab254685 at 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1H antibody (ab254685)Paraffin-embedded human cerebral cortex tissue stained for ATP6V1H using ab254685 at 1/50 dilution in immunohistochemical analysis. -
Western blot - Anti-ATP6V1H antibody (ab254685)All lanes : Anti-ATP6V1H antibody (ab254685) at 0.4 µg/ml
Lane 1 : Vector only transfected HEL-293T cell lysate
Lane 2 : HEK-293T overexpressing ATP6V1H with a C-terminal myc-DDK tag, cell lysate
Predicted band size: 56 kDa
