Anti-RPS6 (phospho S235) antibody - C-terminal (ab227005)
Key features and details
- Rabbit polyclonal to RPS6 (phospho S235) - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RPS6 (phospho S235) antibody - C-terminal
See all RPS6 primary antibodies -
Description
Rabbit polyclonal to RPS6 (phospho S235) - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Dog, Xenopus laevis, Zebrafish
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Immunogen
Synthetic peptide within Human RPS6 (C terminal) (phospho S235). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: P62753 -
Positive control
- IHC-P: Mouse brain tissue. WB: HEK-293T, starved for 16 hours, whole cell extract.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235) antibody - C-terminal (ab227005)
Paraffin-embedded mouse brain tissue stained for RPS6 (phospho S235) using ab227005 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-RPS6 (phospho S235) antibody - C-terminal (ab227005)All lanes : Anti-RPS6 (phospho S235) antibody - C-terminal (ab227005) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), starved for 16 hours, whole cell extract
Lane 2 : HEK-293T, starved for 16 hours and treated with 100 nM Rapamycin for 30 minutes, whole cell extract
Lane 3 : HEK-293T, starved for 16 hours and treated with 100 nM Insulin for 30 minutes, whole cell extract
Lane 4 : HEK-293T, starved for 16 hours and treated with 100 nM Rapamycin for 30 minutes and 100 nM Insulin for 30 minutes, whole cell extract
Lysates/proteins at 40 µg per lane.
Predicted band size: 29 kDa12% SDS-PAGE gel.
