Anti-RPS15 antibody [EPR11104] (ab157193)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11104] to RPS15
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-RPS15 antibody [EPR11104]
See all RPS15 primary antibodies -
Description
Rabbit monoclonal [EPR11104] to RPS15 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide, corresponding to residues in Human RPS15 (UniProt: P62841).
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Positive control
- A549, HepG2, U-87 MG and MCF7 cell lysates; MCF7 and U-87 MG cells; Human brain tissue and Human esophageal carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11104 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-RPS15 antibody [EPR11104] (ab157193) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : U-87 MG cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 17 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS15 antibody [EPR11104] (ab157193)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TPST2 with ab157193 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS15 antibody [EPR11104] (ab157193)
Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma tissue labeling TPST2 with ab157193 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow cytometic analysis of permeabilized U-87 MG cells labeling TPST2 with ab157193 at 1/100 (red) or a rabbit IgG (negative) (green).
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Immunofluorescent analysis of MCF7 cells labeling TPST2 with ab157193 at 1/250 dilution.
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