Anti-ATG10 antibody [EPR4804] (ab124711)
![Anti-ATG10 antibody [EPR4804] (ab124711)](https://www.abcam.com/ps/products/124/ab124711/Images/ab124711-414933-anti-atg10-antibody-epr4804-western-blot-human-knockout-lysate.jpg "Anti-ATG10 antibody [EPR4804] (ab124711)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4804] to ATG10
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-ATG10 antibody [EPR4804]
See all ATG10 primary antibodies -
Description
Rabbit monoclonal [EPR4804] to ATG10 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human ATG10 aa 200-300 (C terminal).
Database link: Q9H0Y0 -
Positive control
- WB: HeLa, K562, and 293T cell lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as Apg10.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 9.20 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4804 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ATG10 antibody [EPR4804] (ab124711)All lanes : Anti-ATG10 antibody [EPR4804] (ab124711) at 1/1000 dilution
Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : ATG10 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab124711 observed at 28 kDa. Red - loading control ab8245 observed at 36 kDa.
ab124711 Anti-ATG10 antibody [EPR4804] was shown to specifically react with ATG10 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265682 (knockout cell lysate ab258786) was used. Wild-type and ATG10 knockout samples were subjected to SDS-PAGE. ab124711 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ATG10 antibody [EPR4804] (ab124711)](https://www.abcam.com/ps/products/124/ab124711/Images/ab124711-138513-anti-atg10-antibody-epr4804-western-blot.jpg)
Western blot - Anti-ATG10 antibody [EPR4804] (ab124711)All lanes : Anti-ATG10 antibody [EPR4804] (ab124711) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : K562 cell lysates
Lane 3 : 293T cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 25 kDa
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![OI-RD Scanning - Anti-ATG10 antibody [EPR4804] (ab124711)](https://www.abcam.com/ps/products/124/ab124711/Images/ab124711-138512-anti-atg10-antibody-epr4804.jpg)
OI-RD Scanning - Anti-ATG10 antibody [EPR4804] (ab124711) -
![Anti-ATG10 antibody [EPR4804] (ab124711)](https://www.abcam.com/ps/products/124/ab124711/Images/ab124711-4-benefits-of-recombinant-antibodies.png)
Anti-ATG10 antibody [EPR4804] (ab124711)
