Itraconazole, Cytochrome p450 inhibitor (ab120816)
Key features and details
- Cytochrome p450 inhibitor
- CAS Number: 84625-61-6
- Purity: > 99%
- Soluble in DMSO to 50 mM and in ethanol to 10 mM (with warming)
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Itraconazole, Cytochrome p450 inhibitor -
Description
Cytochrome p450 inhibitor -
Alternative names
- Oriconazole
- R 51211
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Biological description
Cytochrome p450 CYP3A4 inhibitor (IC50 = 16-26 nM for midazolam hydroxylation). Higher affinity for fungal enzymes over mammalian cytochrome p450. Limited ability to cross the blood-brain barrier. Antifungal activity.
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Purity
> 99% -
CAS Number
84625-61-6 -
Chemical structure
Properties
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Chemical name
4-[4-[4-[4-[[2-(2,4-Dichlorophenyl)-2-(1H-1,2,4-triazol-1-ylmethyl)-1,3-dioxolan-4-yl]methoxy]phenyl]-1-piperazinyl]phenyl]-2,4-dihydro-2-(1-methylpropyl)-3H-1,2,4-triazol-3-one -
Molecular weight
705.64 -
Molecular formula
C35H38Cl2N8O4 -
Storage instructions
Store at -20°C. It is important to note that this product is reported to be light sensitive. Store In the Dark. Store under desiccating conditions. -
Solubility overview
Soluble in DMSO to 50 mM and in ethanol to 10 mM (with warming) -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
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Research areas
Images
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Functional Studies - Itraconazole, Cytochrome p450 inhibitor (ab120816)
HepG2 cells were incubated at 37°C for 24h with vehicle control (0 µM) and different concentrations of itraconazole (ab120816). Increased expression of cytochrome P450 1A1 (ab3568) in HepG2 cells correlates with an increase in nifuroxazide concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab3568 at 1/500 dilution and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.