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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Other ECM Proteins

Anti-Trefoil Factor 3 antibody [EPR3973] - BSA and Azide free (ab247760)

Price and availability

606 422 ₸

Availability

Order now and get it on Thursday June 01, 2023

Anti-Trefoil Factor 3 antibody [EPR3973] - BSA and Azide free (ab247760)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3973] to Trefoil Factor 3 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-Trefoil Factor 3 antibody [EPR3973] - BSA and Azide free
    See all Trefoil Factor 3 primary antibodies
  • Description

    Rabbit monoclonal [EPR3973] to Trefoil Factor 3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247760 is the carrier-free version of ab109104.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3973
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Other ECM Proteins

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR3973] - BSA and Azide free (ab247760)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR3973] - BSA and Azide free (ab247760)
    This data was developed using ab109104, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Trefoil Factor 3 in paraffin-embedded Human colon tissue using ab109104 at 1/500 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Western blot - Anti-Trefoil Factor 3 antibody [EPR3973] - BSA and Azide free (ab247760)
    Western blot - Anti-Trefoil Factor 3 antibody [EPR3973] - BSA and Azide free (ab247760)
    All lanes : Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104) at 1/1000 dilution

    Lane 1 : Human uterus cancer lysate
    Lane 2 : Human colon cancer lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 9 kDa



    This data was developed using ab109104, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-Trefoil Factor 3 antibody [EPR3973] - BSA and Azide free (ab247760)
    Flow Cytometry - Anti-Trefoil Factor 3 antibody [EPR3973] - BSA and Azide free (ab247760)
    This data was developed using ab109104, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of HT-29 (human colorectal adenocarcinoma) cells labeling Trefoil Factor 3 with unpurified ab109104 at 1/100 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
  • Anti-Trefoil Factor 3 antibody [EPR3973] - BSA and Azide free (ab247760)
    Anti-Trefoil Factor 3 antibody [EPR3973] - BSA and Azide free (ab247760)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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