This data was developed using ab177779, the same antibody clone in a different buffer formulation.ab177779 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

Anti-Histone H2B (mono methyl R79) antibody [EPR17612] (ab177779) at 1/50000 dilution + NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 14 kDa
Observed band size: 14 kDa


Exposure time: 1 minute

This data was developed using ab177779, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% BSA /TBST.

Anti-Histone H2B (mono methyl R79) antibody [EPR17612] (ab177779) at 1/50000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 14 kDa
Observed band size: 14 kDa


Exposure time: 1 minute

This data was developed using ab177779, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% BSA /TBST.