Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free (ab247549)
![Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free (ab247549)](https://www.abcam.com/ps/products/247/ab247549/Images/ab247549-407978-anti-calpain-small-subunit-1-antibody-epr3323-bsa-and-azide-free-western-blot.jpg "Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free (ab247549)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3323] to Calpain small subunit 1 - BSA and Azide free
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free
See all Calpain small subunit 1 primary antibodies -
Description
Rabbit monoclonal [EPR3323] to Calpain small subunit 1 - BSA and Azide free -
Host species
Rabbit -
Specificity
Detects Calpain small subunit 1.
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Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab247549 is the carrier-free version of ab92356. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247549 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR3323 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free (ab247549)All lanes : Anti-Calpain small subunit 1 antibody [EPR3323] (ab92356) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CAPNS1 (Calpain small subunit 1) knockout HAP1 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 28 kDaThis data was developed using ab92356, the same antibody clone in a different buffer formulation.
Lanes 1 - 4: Merged signal (red and green). Green - ab92356 observed at 82 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab92356 was shown to specifically react with Calpain 1 in wild-type HAP1 cells as signal was lost in CAPNS1 (Calpain small subunit 1) knockout cells. Wild-type and CAPNS1 (Calpain small subunit 1) knockout samples were subjected to SDS-PAGE. ab92356 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free (ab247549)](https://www.abcam.com/ps/products/247/ab247549/Images/ab247549-402491-anti-calpain-small-subunit-1-antibody-epr3323-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free (ab247549)All lanes : Anti-Calpain small subunit 1 antibody [EPR3323] (ab92356) at 1/1000 dilution
Lane 1 : T47-D cell lysate
Lane 2 : 293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution
Predicted band size: 28 kDaThis data was developed using ab92356, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free (ab247549)](https://www.abcam.com/ps/products/247/ab247549/Images/ab247549-1-anti-calpain-small-subunit-1-antibody-epr3323-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free (ab247549)This data was developed using ab92356, the same antibody clone in a different buffer formulation.ab92356 at 1/100 dilution staining Calpain small subunit 1 in paraffin-embedded Human kidney Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. -
![Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free (ab247549)](https://www.abcam.com/ps/products/247/ab247549/Images/ab247549-4-benefits-of-recombinant-antibodies.png)
Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free (ab247549)