Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free (ab247549)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3323] to Calpain small subunit 1 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free
    See all Calpain small subunit 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3323] to Calpain small subunit 1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Detects Calpain small subunit 1.

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247549 is the carrier-free version of ab92356. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247549 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free (ab247549)
    Western blot - Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free (ab247549)
    All lanes : Anti-Calpain small subunit 1 antibody [EPR3323] (ab92356) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : CAPNS1 (Calpain small subunit 1) knockout HAP1 whole cell lysate
    Lane 3 : HepG2 whole cell lysate
    Lane 4 : HeLa whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 28 kDa



    This data was developed using ab92356, the same antibody clone in a different buffer formulation.

    Lanes 1 - 4: Merged signal (red and green). Green - ab92356 observed at 82 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab92356 was shown to specifically react with Calpain 1 in wild-type HAP1 cells as signal was lost in CAPNS1 (Calpain small subunit 1) knockout cells. Wild-type and CAPNS1 (Calpain small subunit 1) knockout samples were subjected to SDS-PAGE. ab92356 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free (ab247549)
    Western blot - Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free (ab247549)
    All lanes : Anti-Calpain small subunit 1 antibody [EPR3323] (ab92356) at 1/1000 dilution

    Lane 1 : T47-D cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution

    Predicted band size: 28 kDa



    This data was developed using ab92356, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free (ab247549)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free (ab247549)
    This data was developed using ab92356, the same antibody clone in a different buffer formulation.ab92356 at 1/100 dilution staining Calpain small subunit 1 in paraffin-embedded Human kidney Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free (ab247549)
    Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free (ab247549)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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