Anti-CD47 antibody (ab175388)
Key features and details
- Rabbit polyclonal to CD47
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CD47 antibody
See all CD47 primary antibodies -
Description
Rabbit polyclonal to CD47 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Fusion protein corresponding to Human CD47 aa 1-150.
Database link: Q08722 -
Positive control
- WB: MCF-7 and SK-OV-3 cell lysates; Mouse lung lysate. IHC: human placenta and human lung cancer tissues. ICC/IF: L929 and U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody (ab175388)
Formalin-fixed, paraffin-embedded human placenta tissue stained for CD47 using ab175388 at 1/200 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-CD47 antibody (ab175388)Immunofluorescence staining of L929 cells stained for CD47 with ab175388 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
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Western blot - Anti-CD47 antibody (ab175388)All lanes : Anti-CD47 antibody (ab175388) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : SK-OV-3 (human ovary adenocarcinoma cell line) cell lysate
Lane 3 : Mouse lung lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 35 kDa
Exposure time: 90 secondsBlocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody (ab175388)Formalin-fixed, paraffin-embedded human lung cancer tissue stained for CD47 using ab175388 at 1/200 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-CD47 antibody (ab175388)Immunofluorescence staining of U-2 OS cells stained for CD47 with ab175388 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
