Anti-p53 antibody [PAb421] (ab245685)
Key features and details
- Rabbit monoclonal [PAb421] to p53
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-p53 antibody [PAb421]
See all p53 primary antibodies -
Description
Rabbit monoclonal [PAb421] to p53 -
Host species
Rabbit -
Specificity
This antibody recognizes the mammalian mutant and wild-type p53 protein.
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Tested applications
Suitable for: Flow Cyt, WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey, Rhesus monkey -
Immunogen
Synthetic peptide corresponding to Human p53 aa 350 to the C-terminus.
Database link: P04637 -
Positive control
- Flow Cyt: Jurkat cells. WB: A431 nuclear and cytoplasmic lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
PAb421 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry ab245685.
Jurkat (Human T cell leukemia cell line from peripheral blood) cells were stained with unimmunized rabbit IgG antibody (black line) or ab245685 (blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 µg/ml.
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All lanes : Anti-p53 antibody [PAb421] (ab245685) at 0.1 µg/ml (incubated for 1 hr)
Lane 1 : A431 (Human epidermoid carcinoma cell line) nuclear lysate (in RIPA buffer)
Lane 2 : A431 cytoplasmic lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.10% SDS PAGE gel.
Detected by chemiluminescence.
The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651).