Anti-EEF2K (phospho S359) antibody [EPR2252(N)] - BSA and Azide free (ab251126)
![Anti-EEF2K (phospho S359) antibody [EPR2252(N)] - BSA and Azide free (ab251126)](https://www.abcam.com/ps/products/251/ab251126/Images/ab251126-405587-anti-eef2k-phospho-s359-antibody-epr2252-n-bsa-and-azide-free-western-blot.jpg "Anti-EEF2K (phospho S359) antibody [EPR2252(N)] - BSA and Azide free (ab251126)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2252(N)] to EEF2K (phospho S359) - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-EEF2K (phospho S359) antibody [EPR2252(N)] - BSA and Azide free
See all EEF2K primary antibodies -
Description
Rabbit monoclonal [EPR2252(N)] to EEF2K (phospho S359) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251126 is the carrier-free version of ab192257. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251126 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR2252(N) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EEF2K (phospho S359) antibody [EPR2252(N)] - BSA and Azide free (ab251126)All lanes : Anti-EEF2K (phospho S359) antibody [EPR2252(N)] (ab192257) at 1/1000 dilution
Lane 1 : HeLa cell lysate, treated with Okadaic Acid + Calyculin A
Lane 2 : HeLa cell lysate, untreated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 82 kDaThis data was developed using ab192257, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K (phospho S359) antibody [EPR2252(N)] - BSA and Azide free (ab251126)](https://www.abcam.com/ps/products/251/ab251126/Images/ab251126-2-anti-eef2k-phospho-s359-antibody-epr2252-n-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K (phospho S359) antibody [EPR2252(N)] - BSA and Azide free (ab251126)This data was developed using ab192257, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling EEF2K with ab192257 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K (phospho S359) antibody [EPR2252(N)] - BSA and Azide free (ab251126)](https://www.abcam.com/ps/products/251/ab251126/Images/ab251126-1-anti-eef2k-phospho-s359-antibody-epr2252-n-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K (phospho S359) antibody [EPR2252(N)] - BSA and Azide free (ab251126)This data was developed using ab192257, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling EEF2K with ab192257 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Anti-EEF2K (phospho S359) antibody [EPR2252(N)] - BSA and Azide free (ab251126)](https://www.abcam.com/ps/products/251/ab251126/Images/ab251126-4-benefits-of-recombinant-antibodies.png)
Anti-EEF2K (phospho S359) antibody [EPR2252(N)] - BSA and Azide free (ab251126)