Anti-p53 (phospho S15) antibody (ab38497)
Key features and details
- Rabbit polyclonal to p53 (phospho S15)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-p53 (phospho S15) antibody
See all p53 primary antibodies -
Description
Rabbit polyclonal to p53 (phospho S15) -
Host species
Rabbit -
Specificity
This antibody is specific for p53 only when phosphorylated at serine 15. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human p53 (phospho S15). Synthetic phosphopeptide derived from Human p53 around the phosphorylation site of serine 15.
Database link: P04637
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-p53 (phospho S15) antibody (ab38497)All lanes : Anti-p53 (phospho S15) antibody (ab38497)
Lane 1 : HeLa cells extract (5-30ug of total protein).
Lane 2 : HeLa cells extract (5-30ug of total protein). Treated with hydroxyurea
Predicted band size: 53 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S15) antibody (ab38497)ab38497 (1/100) staining p53 (phospho S15) in paraffin-embedded human breast carcinoma sections. Right-hand panel corresponds to a negative control where ab38497 was pre-incubated with the immunizing (blocking) peptide.
