Cathepsin K Inhibitor Assay Kit (Fluorometric) (ab185439)
Key features and details
- Assay type: Semi-quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Inhibitor compounds
Overview
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Product name
Cathepsin K Inhibitor Assay Kit (Fluorometric)
See all Cathepsin K kits -
Detection method
Fluorescent -
Sample type
Inhibitor compounds -
Assay type
Semi-quantitative -
Product overview
Cathepsin K Inhibitor Screening Kit (ab185439) uses the ability of active Cathepsin K to cleave the synthetic AFC based peptide substrate torelease AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin K-specific inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of Cathepsin K.
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Notes
Cathepsin K (CTSK, EC 3.4.22.38), a lysosomal cysteine protease, is involved in osteoclastic bone remodeling and resorption. In addition, it also degrades collagen, gelatin and elastin.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 100 tests Cathepsin K 1 vial CTS K Inhibitor (FF-FMK, 1 mM) 1 x 20µl CTS K Reaction Buffer 1 x 15ml CTS K Reagent 1 x 100µl CTS K Substrate (Ac-LR-AFC 10 mM) 1 x 0.2ml -
Research areas
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Relevance
Cathepsin K is closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. -
Cellular localization
Lysosome. -
Alternative names
- Cathepsin O
- Cathepsin O2
- Cathepsin X
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Images
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Inhibition of Cathepsin K activity by the Cathepsin K Inhibitor [FF-FMK]
Inhibition of Cathepsin K activity by Cathepsin K Inhibitor Screening Kit was performed following kit protocol. This is example data only.
