Cathepsin K Activity Assay Kit (Fluorometric) (ab65303)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell Lysate, Tissue Extracts
Overview
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Product name
Cathepsin K Activity Assay Kit (Fluorometric)
See all Cathepsin K kits -
Detection method
Fluorescent -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Enzyme activity -
Assay time
2h 00m -
Product overview
Cathepsin K Activity Assay Kit (Fluorometric) (ab65303) is a fluorescence-based assay that utilizes the preferred cathepsin K substrate sequence LR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin K will cleave the synthetic substrate LR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.
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Notes
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests CK Cell Lysis Buffer WM 1 x 25ml CK Inhibitor (1mM) Red 1 x 20µl CK Reaction Buffer NM 1 x 5ml CK Substrate Ac-LR-AFC (10mM) Amber 1 x 200µl -
Research areas
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Relevance
Cathepsin K is closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. -
Cellular localization
Lysosome. -
Alternative names
- Cathepsin O
- Cathepsin O2
- Cathepsin X
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Images
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ab65303 Cathepsin K Activity
Cathepsin K Activity measured in tissue lysates (each with protein concentration of 4 mg/mL) after 1 hour of incubation with and without inhibitor (I).
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ab65303 Cathepsin K ActivityCathepsin K Activity measured in cell lysates (each with cells concentration of 4e7 cells/mL) after 1 hour of incubation with and without inhibitor (I).
