Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD33 antibody [EPR23052-5] - BSA and Azide free (ab264572)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23052-5] to CD33 - BSA and Azide free
  • Suitable for: IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD33 antibody [EPR23052-5] - BSA and Azide free
    See all CD33 primary antibodies

  • Description

    Rabbit monoclonal [EPR23052-5] to CD33 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Jurkat and Human peripheral blood mononuclear cell cells. IP: THP-1 cells.

  • General notes

    ab264572 is the carrier-free version of ab264558. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-CD33 antibody [EPR23052-5] - BSA and Azide free (ab264572)
    Immunoprecipitation - Anti-CD33 antibody [EPR23052-5] - BSA and Azide free (ab264572)
    CD33 was immunoprecipitated from 0.35 mg THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10ug with ab264558 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab264558 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10ug

    Lane 2: ab264558 IP in THP-1 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab264558 in THP-1 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 min

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264558).

  • Flow Cytometry - Anti-CD33 antibody [EPR23052-5] - BSA and Azide free (ab264572)
    Flow Cytometry - Anti-CD33 antibody [EPR23052-5] - BSA and Azide free (ab264572)

    Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD33 with ab264558 at 1/50 compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. To be safe, we recommend 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264558).

  • Flow Cytometry - Anti-CD33 antibody [EPR23052-5] - BSA and Azide free (ab264572)
    Flow Cytometry - Anti-CD33 antibody [EPR23052-5] - BSA and Azide free (ab264572)

    Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / THP-1 (Human monocytic leukemia monocyte, Right) cells labelling CD33 with ab264558 at 1/50 compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264558).

  • Anti-CD33 antibody [EPR23052-5] - BSA and Azide free (ab264572)
    Anti-CD33 antibody [EPR23052-5] - BSA and Azide free (ab264572)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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