Anti-CD33 antibody [BLR061G] - BSA free (ab272082)
![Anti-CD33 antibody [BLR061G] - BSA free (ab272082)](https://www.abcam.com/ps/products/272/ab272082/Images/ab272082-1-ab264414365933anticd33antibodyimmunoprecipitationhel921celllysate.jpg "Anti-CD33 antibody [BLR061G] - BSA free (ab272082)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR061G] to CD33 - BSA free
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CD33 antibody [BLR061G] - BSA free
See all CD33 primary antibodies -
Description
Rabbit monoclonal [BLR061G] to CD33 - BSA free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human CD33 aa 314-364 (C terminal). The exact sequence is proprietary.
Database link: P20138 -
Positive control
- IHC-P: Acute Myeloid leukemia tissue. IP: HEL921.7 whole cell lysate.
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General notes
This product is sold under License from Bethyl Laboratories, Inc.
ab272082 is the carrier-free version of 264414. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading... -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR061G -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-CD33 antibody [BLR061G] - BSA free (ab272082)
CD33 was immunoprecipitated from HEL 92.1.7 whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264414 at 20 µl/mg lysate. Western blot was performed on the immunoprecipitate using ab264414 at 1/1000 dilution.
Lane 1: ab264414 IP in HEL 92.1.7 cell lysate.
Lane 2: Control IgG IP in HEL 92.1.7 cell lysate.Detection: Chemiluminescence with an exposure time of 10 seconds.
Lysates prepared using NETN lysis buffer.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264414).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [BLR061G] - BSA free (ab272082)](https://www.abcam.com/ps/products/272/ab272082/Images/ab272082-2-ab2644141anticd33antibodyimmunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [BLR061G] - BSA free (ab272082)Formalin-fixed, paraffin-embedded Acute Myeloid Leukemia tissue stained for CD33 using ab264414 at 1/200 dilution in immunohistochemical analysis. The secondary was an HRP-conjugated goat anti-rabbit IgG antibody. Substrate: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264414).
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![Anti-CD33 antibody [BLR061G] - BSA free (ab272082)](https://www.abcam.com/ps/products/272/ab272082/Images/ab272082-3-benefits-of-recombinant-antibodies.png)
Anti-CD33 antibody [BLR061G] - BSA free (ab272082)
