Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD33 antibody [WM53] - BSA and Azide free (ab252263)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [WM53] to CD33 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD33 antibody [WM53] - BSA and Azide free
    See all CD33 primary antibodies

  • Description

    Mouse monoclonal [WM53] to CD33 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Human peripheral blood mononuclear cells (PBMC). ICC: HL-60 cells

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab252263 is the carrier-free version of ab30371. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CD33 antibody [WM53] - BSA and Azide free (ab252263)
    Immunocytochemistry/ Immunofluorescence - Anti-CD33 antibody [WM53] - BSA and Azide free (ab252263)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab30371)

    ab30371 staining CD33 in HL-60 cells. The cells were fixed with 100% MeOH (5min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab30371 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).

     

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Flow Cytometry - Anti-CD33 antibody [WM53] - BSA and Azide free (ab252263)
    Flow Cytometry - Anti-CD33 antibody [WM53] - BSA and Azide free (ab252263)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) (right) labeling CD33 with ab30371 at 1/40 dilution compared with mouse monoclonal IgG Isotype Control (left). Goat anti-mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with mouse IgG (Left) or ab30371 (Right). Then stained with anti-CD14 conjugated to Alexa Fluor® 647.

    Gated on viable cells.

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab30371).

  • Anti-CD33 antibody [WM53] - BSA and Azide free (ab252263)
    Anti-CD33 antibody [WM53] - BSA and Azide free (ab252263)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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