Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ILKAP antibody [EPR16145] (ab196013)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16145] to ILKAP
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ILKAP antibody [EPR16145]
    See all ILKAP primary antibodies

  • Description

    Rabbit monoclonal [EPR16145] to ILKAP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, HAP1, HeLa, 293, Mouse brain and Rat brain lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16145

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    All lanes : Anti-ILKAP antibody [EPR16145] (ab196013) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : ILKAP knockout HEK293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HAP1 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 43 kDa
    Observed band size: 49 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab196013 observed at 49 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab196013 Anti-ILKAP antibody [EPR16145] was shown to specifically react with ILKAP in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266475 (knockout cell lysate ab258000) was used. Wild-type and ILKAP knockout samples were subjected to SDS-PAGE. ab196013 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-ILKAP antibody [EPR16145] (ab196013)
    Immunoprecipitation - Anti-ILKAP antibody [EPR16145] (ab196013)

    Immunoprecipitation analysis of 293 (Human epithelial cells from embryonic kidney) whole cell extract labeling ILKAP using ab196013 at 1/30 dilution (Lane 2).
    Lane 3: IP using Rabbit monoclonal IgG (ab172730) instead of ab196013 in 293 (Human epithelial cells from embryonic kidney) whole cell extract.
    Lane 1: Input: 10 μg 293 (Human epithelial cells from embryonic kidney) whole cell extract.
    Subsequent WB detection was performed using ab196013 at 1/1000 dilution.
    An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

  • Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: ILKAP knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HEK293 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab196013 observed at 43 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab196013 was shown to recognize ILKAP when ILKAP knockout samples were used, along with additional cross-reactive bands. Wild-type and ILKAP knockout samples were subjected to SDS-PAGE. Ab196013 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    Anti-ILKAP antibody [EPR16145] (ab196013) at 1/20000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa

  • Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    Anti-ILKAP antibody [EPR16145] (ab196013) at 1/20000 dilution + 293 (Human epithelial cells from embryonic kidney) cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa

  • Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    Anti-ILKAP antibody [EPR16145] (ab196013) at 1/5000 dilution + Mouse brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa

  • Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    Anti-ILKAP antibody [EPR16145] (ab196013) at 1/1000 dilution + Rat brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa

  • Anti-ILKAP antibody [EPR16145] (ab196013)
    Anti-ILKAP antibody [EPR16145] (ab196013)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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